TABLE 3.

Quinolone susceptibilities of and topoisomerase changes in S. aureus clinical isolates

Organism	Strain no.	Results of RFLP analysisa		Protein change				MIC (μg/ml)
		grlA	gyrA	GyrA	GyrB	GrlA	GrlB	Trovafloxacin	Ciprofloxacin	Levofloxacin	Sparfloxacin	Pefloxacin
MSSA	1081	79/80	None	None	None	None	Glu422 to Asp	0.031	0.25	0.25	0.125	0.5
MSSA	1039	79/80	None	None	None	Ser80 to Phe	None	0.125	1	1	0.125	4
MSSA	1066	84	None	None	None	Glu84 to Lys	Ser410 to Pro, Glu422 to Asp	0.125	2	0.5	0.25	4
MSSA	1035	79/80	None	None	None	Ser80 to Phe	None	0.125	1	1	0.25	8
MSSA	1249	79/80	None	None	None	Ser80 to Phe	None	0.125	2	0.5	0.25	8
MSSA	1305	84	None	None	None	Glu84 to Lys	None	0.125	1	1	0.25	8
MSSA	1134	79/80	None	None	None	Ser80 to Tyr	None	0.25	1	1	0.25	8
MSSA	872	84	None	None	None	Glu84 to Lys	Glu422 to Asp	0.125	1	0.5	0.125	4
MSSA	1642	79/80	None	None	None	Ser80 to Phe	None	0.25	2	1	0.25	8
MSSA	1275	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	His478 to Tyr	1	8	8	8	64
MSSA	1640	79/80	83/84	Ser84 to Leu	None	Ser80 to Tyr	None	1	8	4	16	64
MSSA	1637	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	His478 to Tyr	4	32	16	32	128
MSSA	1628	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	Glu422 to Asp	4	256	16	8	256
MRSA	906	79/80	83/84	Ser84 to Leu	None	Ser80 to Tyr	Glu422 to Asp	1	8	8	16	128
MRSA	970	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	Asp432 to Val	1	32	32	16	128
MRSA	954	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	None	2	64	8	16	128
MRSA	1326	79/80	83/84	Ser84 to Leu	None	Ser80 to Tyr	Glu422 to Asp	2	32	8	16	128
MRSA	1617	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	None	2	32	8	16	128
MRSA	1623	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	None	1	16	4	16	128
MRSA	1626	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	None	2	32	8	16	256
MRSA	1000	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	Glu422 to Asp	1	32	8	16	128
MRSA	1020	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	Glu422 to Asp	2	128	16	16	128
MRSA	1629	79/80	83/84	Ser84 to Leu	None	Ser80 to Tyr	None	1	8	8	16	128
MRSA	1630	79/80	83/84	Ser84 to Leu	None	Ser80 to Tyr	None	1	16	4	8	64
MRSA	1627	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	None	2	32	8	16	256
MRSA	1151	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	None	1	32	8	8	256
MRSA	1254	79/80	83/84	Ser84 to Leu	None	Ser80 to Phe	Glu422 to Asp	8	256	32	32	256
MRSA	1643	79/80	83/84	Ser84 to Leu	None	Ser80 to Tyr	Glu422 to Asp	8	64	32	32	128
MRSA	983	79/80	83/84	Ser84 to Leu	None	Ser80 to Tyr, Glu84 to Lys	Glu422 to Asp	16	128	64	32	256

^aCodon(s) in which a mutation(s) resulting in loss or acquisition of recognition sites was found.