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. 2022 Mar 17;14:1165–1177. doi: 10.2147/CMAR.S335749

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© 2022 Wang et al.

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Knockdown of GPR27 inhibits HCC cells proliferation in vitro. (A) The mRNA and protein expression of GPR27 were examined in HCC cell lines. (B and C) GPR27 knockdown efficiency of siRNAs was determined by qRT-PCR and Western blot analysis. (D) Colony formation assays were performed to determine the cell clonogenicity of SMMC-7721 and SK-Hep-1 cells treated with control or GPR27 siRNAs. (E) The effects of GPR27 on cell proliferation of SMMC-7721 and SK-Hep-1 cells were detected by measuring the intracellular ATP content treated with control or GPR27 siRNAs. (F) Real Time Cellular Analysis (RTCA) assays were performed to evaluate the effect of GPR27 on HCC cell proliferation treated with control or GPR27 siRNAs. Data are represented as the mean ± SD of three independent experiments. The p-values < 0.05 were considered statistically significant for all tests.