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. 2022 Mar 17;14:1165–1177. doi: 10.2147/CMAR.S335749

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© 2022 Wang et al.

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Overexpression of GPR27 promotes HCC cells proliferation in vitro. (A) GPR27 overexpression efficiency was determined by qRT-PCR and Western blot analysis after lentiviral infection in SMMC-7721 and SK-Hep-1 cells. (B) Colony formation assays were performed to determine the cell clonogenicity of GPR27-OE SMMC-7721 and SK-Hep-1 cells. (C) Growth curve analysis was conducted to examine the cell proliferation in GPR27-OE SMMC-7721 and SK-Hep-1 cells. Data are represented as the mean ± SD of three independent experiments. The p-values < 0.05 were considered statistically significant for all tests.