Figure 8.

CircHGF served as a sponge for miR-25-3p and miR-25-3p could directly target SMAD7 3′UTR

(A) The binding sequence between circHGF and miR-25-3p as predicted by bioinformatics analysis based on the starBase online database. (B) The binding site between SMAD7 and miR-25-3p as predicted by bioinformatics analysis based on the TargetScan 7 online tool and miRDB online database. (C) Relative luciferase reporter activity of WT or MUT circHGF in 293T cells co-transfected with miR-25-3p mimics or the control mimics NC. (D) Relative luciferase reporter activity of WT or MUT SMAD7 3′UTR in 293T cells co-transfected with miR-25-3p mimics or mimics NC. (E) The WT SMAD7 3′UTR luciferase reporter vector was co-transfected into 293T cells with miR-25-3p mimics or mimics NC, and OE-circRNA or OE-NC vector, and the luciferase activity was detected in corresponding groups. Data were shown as means with error bars representing SD. ^∗∗p < 0.01, n = 3. Data between two groups were analyzed by Student's t test. Data among multiple groups were analyzed by one-way ANOVA test.