Table 2.
Descriptive statistics of nicotine biomarkers on Day 3 by group (N = 10 subjects in each group).
| Biomarker | NU | CC | EC | HTP | NRT | OT | |
|---|---|---|---|---|---|---|---|
| Nequ (Nic + 10) (mg/24 h) | Mean ± SD | 0.06 ± 0.09 | 16.87 ± 9.66 | 6.79 ± 4.92 | 12.47 ± 6.80 | 6.53 ± 6.35 | 22.10 ± 12.73 |
| Median (IQR)3 | 0.02 (0.01–0.07) | 11.03 (9.92–25.24) | 5.62 (3.55–7.75) | 13.89 (5.41–18.80) | 3.41 (2.07–9.31) | 23.26 (11.82–28.03) | |
| Min–Max | 0.01–0.27 | 9.51–33.86 | 3.09–19.64 | 1.73–20.74 | 1.64–21.63 | 4.53–49.94 | |
| Different from | CC,EC,HTP,NRT,OT | NU,EC,NRT | NU,CC,OT | NU | NU,CC,OT | NU,EC,NRT | |
| Plasma nicotine1(ng/mL) | Mean ± SD | 0.05 ± 0.07 | 19.2 ± 13.1 | 9.1 ± 7.2 | 12.8 ± 8.9 | 9.4 ± 8.1 | 19.1 ± 7.9 |
| Median (IQR)3 | 0.00 (0.00–0.10) | 21.0 (4.3–28.4) | 7.4 (3.6–12.5) | 13.9 (5.3–19.5) | 6.5 (2.3–16.4) | 19.6 (11.6–27.4) | |
| Min–Max | 0.00–0.20 | 2.8–39.0 | 2.5–26.7 | 0.02–27.4 | 0.3–21.9 | 7.3–30.9 | |
| Different from | CC,EC,HTP,NRT,OT | NU | NU | NU | NU | NU | |
| Plasma cotinine1(ng/mL) | Mean ± SD | 0.09 ± 0.03 | 256.3 ± 124.2 | 159.1 ± 106.9 | 189.4 ± 94.3 | 154.5 ± 128.8 | 354.8 ± 141.1 |
| Median (IQR)3 | 0.10 (0.10–0.10) | 257.5 (116.1–367.6) | 125.0 (87.1–203.1) | 217.2 (106.4–252.1) | 121.3 (46.5–283.7) | 371.2 (225.0–578.3) | |
| Min–Max | 0.00–0.10 | 114.7–466.7 | 73.2–430.9 | 16.1–301.8 | 133.2–385.3 | 136.1–578.3 | |
| Different from | CC, EC, HTP, NRT, OT | NU | NU,OT | NU | NU,OT | NU,EC,NRT | |
| Plasma 3-OH-Cot1(ng/mL) | Mean ± SD | 0.07 ± 0.12 | 60.6 ± 30.3 | 48.7 ± 34.5 | 53.4 ± 26.4 | 39.4 ± 37.81 | 90.5 ± 49.3 |
| Median (IQR)3 | 0.00 (0.00–0.20) | 56.4 (39.4–119.1) | 43.2 (20.6–128.4) | 56.5 (38.3–80.4) | 34.3 (14.5–49.5) | 75.7 (43.9–139.9) | |
| Min–Max | 0.00–0.30 | 12.4–119.1 | 16.8–128.4 | 8.8–82.2 | 1.0–134.5 | 38.3–158.2 | |
| Different from | CC,EC,HTP,NRT,OT | NU | NU | NU | NU,OT | NU,NRT | |
| Saliva cotinine2(ng/mL) | Mean ± SD | 0.17 ± 0.13 | 144.2 ± 79.7 | 102.1 ± 51.9 | 168.1 ± 125.3 | 127.5 ± 122.1 | 267.7 ± 106.8 |
| Median (IQR)3 | 0.21 (0.04–0.26) | 127.0 (83.0–210.5) | 84.9 (60.7–136.2) | 174.2 (43.7–292.1) | 108.8 (35.2–167.0) | 264.9 (191.0–350.3) | |
| Min–Max | 0.00–0.37 | 41.3–271.8 | 51.6–217.8 | 1.76–353.4 | 27.3–388.8 | 97.7–431.0 | |
| Different from | CC,EC,HTP,NRT,OT | NU | NU,OT | NU | NU,OT | NU,EC,NRT | |
| Saliva 3-OH-Cot2(ng/mL) | Mean ± SD | 0.05 ± 0.12 | 32.1 ± 22.0 | 26.7 ± 14.1 | 31.6 ± 19.8 | 34.4 ± 41.5 | 49.2 ± 21.5 |
| Median (IQR)3 | 0.00 (0.00–0.02) | 26.7 (15.8–45.5) | 33.0 (10.2–39.0) | 33.2 (11.8–46.9) | 14.8 (8.9–54.3) | 46.6 (27.6–67.1) | |
| Min–Max | 0.00–0.37 | 9.5–83.2 | 5.6–41.3 | 1.0–61.8 | 0.7–134.4 | 21.9–82.1 | |
| Different from | CC,EC,HTP,NRT,OT | NU | NU | NU | NU | NU | |
| Estimated nicotine intake4(mg/d) | Mean ± SD | 6.17 ± 0.76 | 9.38 ± 5.63 | 4.49 ± 2.30 | 7.70 ± 4.67 | 17.31 ± 10.28 | |
| Median (IQR)3 | 6.22 (4.18–7.84) | 8.39 (5.12–11.51) | 4.56 (2.62–7.09) | 7.00 (3.50–10.50) | 15.57 (10.27–20.54) | ||
| Min–Max | 2.03–10.27 | 4.41–23.31 | 0.34–7.43 | 2.80–16.80 | 3.66–39.37 | ||
| Different from | OT | HTP |
1
Plasma sample from Day 3 at 5 pm (B6, Fig. 1).
2
Saliva sample from Day 3 at 5 pm (S6).
3
IQR: Inter quartile range (25th −75th percentile).
4
Calculated for Day 3 by applying the models described in Section 2.3.