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. 2022 Mar 18;3(2):101187. doi: 10.1016/j.xpro.2022.101187

Program the following protocol on a real-time PCR thermocycler:

Step Cycles Temperature Time
1 1 95°C 30 s
2 40 95°C 30 s
3 40 60°C 10 s
4 Melting curve variable
5 1 4°C keep