Fig. 2. Preprocessing, consistency, and categorization of FRSs.

a Illustration of the four filters applied to perturbed sequences to remove inactive and non-functional sites, both at each timepoint and across timepoints (error bars represent mean ± 1 SD; “Methods”). b Number of sequences that passed all three filters for each perturbation method. c Definition of main and sub-categories of motif binding effects based on their effect on transcription. d Distribution of categories across FRSs that pass the four filters and are under the same main category (activating or dampening) in perturbation method 3 and at least one of the perturbation methods 1 or 2. The distribution is shown across FRSs (top) and across the unique motifs and regions composing the FRSs in this study (bottom).