Figure 3.

RanGAP formed granular inclusions in cells expressing DPRs. (A–E) HeLa cells transfected with GFP-tagged DPR constructs or empty pEGFP-N1 vector as a GFP-only control were fixed and stained 48 h post-transfection. RanGAP (red) is diffusely expressed in the nucleus of GFP-only control cells (A) but formed granular inclusions in the nuclei of a proportion of cells expressing DPRs (B–E). Scale bars indicate 15 μm. (F) Graph shows the percentage of cells exhibiting granular RanGAP inclusions, which was significantly increased by expression of GA₁₀₂₀ (P = 0.0483), GR₁₁₃₆ (P < 0.0001), PR₁₁₀₀ (P = 0.0002) or AP₁₀₂₄ (P = 0.0096). n = 3, with a minimum of 30 cells analysed for each independent replicate. Data was analysed by one-way ANOVA with Dunnett’s multiple comparison test. Error bars indicate SEM.