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. 2022 Mar 21;13:1493. doi: 10.1038/s41467-022-29240-5

Fig. 2. cAMP-CRP complex supports biofilm maintenance in S. putrefaciens CN32.

Fig. 2

a Biofilm biomass (n = 4 independent samples). b Cell growth (n = 3 independent samples). c Intracellular cAMP concentration at 30 h (n = 3 independent samples). d Biofilm biomass at 30 h (n = 6 independent samples). e Intracellular cAMP concentration with the addition of 1 mM exogenous cAMP to the culture medium vs. control (no addition of exogenous cAMP [0 mM]) at 30 h (n = 3 independent samples). f Biofilm biomass with the addition of 1 mM exogenous cAMP to the culture medium vs. control at 30 h (n = 3 independent samples). Insets in (a, d, f) are the biofilm pictures of crystal violet dyeing. Data in (a–f) are shown as the mean ± SD. Two-sided Student’s t test was used in (a, c–f) to analyze the statistical significance (NS: No significance. ***p < 0.001). Source data are provided as a Source Data file.