Fig. 6. cAMP is not necessary for physical interaction between CRP and BpfD, but it is essential to help CRP enhance the interaction between BpfD and BpfG.
a GST pull-down assay showing the interaction between BpfD intracellular domains and CRP-R84L in vitro. b Biofilm biomass (n = 3 independent samples). c Co-IP to analyze the interaction between BpfD and BpfG in vivo. d Western blotting detection of BpfA localization on the cell surface. All strains used in (a, c, d) were cultured for 30 h in the biofilm state. The native promoter region of the bpfA operon of the strains used in (c, d) was replaced by the constitutive promoter PaacC1. Data in (a) are shown as the mean ± SD. Two-sided Student’s t test was used in (a) to analyze the statistical significance (NS: No significance. ***p < 0.001). Source data are provided as a Source Data file.