Figure 3.

Cell stem cell transcriptional factors SOX2 and OCT4 bind with promoter and upregulate the expression of HNRNPM.A, The basic expression of HNRNPM in different HCC cell lines. B-C, Western blot analysis of HNRNPM expression when overexpressing (B) or depletion of (C) OCT4 and SOX2. D, The predicted binding site for OCT4 and SOX2 with HNRNPM promoter. E, OCT4 directly binds with HNRNPM promoter by ChIP assays and luciferase assays. F, SOX2 directly binds with HNRNPM promoter by ChIP assays and luciferase assays. G-H, Correlation analysis between OCT4 (G), SOX2 (H), and HNRNPM from TCGA database.