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. 2022 Feb 12;13(5):1413–1447. doi: 10.1016/j.jcmgh.2022.02.006

Figure 4.

Figure 4

The tumorigenesis effects of HNRNPM overexpression in MHCC97L and HepG2 cells.A-B, The mRNA and protein levels of HNRNPM in MHCC97L (A) and HepG2 cells (B) stably overexpressing HNRNPM. C-D, The cell proliferation by CCK-8 assays stably MHCC97L (C) and HepG2 cells (D) stably overexpressing HNRNPM. ∗∗∗∗P < .0001 as compared with control. E-F, The cell apotosis by flow cytometry stably MHCC97L (E) and HepG2 cells (F) stably overexpressing HNRNPM. Data were from 3 independent experiments. ∗∗P < .01 by the Student t test. G-H, The in vivo effects in BALB/c nude mice in MHCC97L (G; n = 6) and HepG2 cells (H; n = 6) stably overexpressing HNRNPM. ∗∗P < .01 by the Student t test. I, The CSC frequency was determined from a limiting dilution assay performed with HCC cells depleting HNRNPM from the third transplant recipient mice (n = 6). The ELDA web tool was used to calculate the frequency of CSCs.