Figure 7.

HNRNPM is required for tumorigenesis of HCC cells.A, The mRNA and protein levels of HNRNPM in MHCC97H cells stably depleting HNRNPM. B, The protein levels of HNRNPM by immunofluorence stably depleting HNRNPM. C, Sphere formation and limiting dilution assays when depleting HNRNPM in MHCC97H cells. The number of spheroids formed as a fraction of the number of cells seeded per well is given. Data are from 3 independent experiments. ∗∗P < .01 by the Student t test. D, The cell proliferation by CCK-8 assays stably depleting HNRNPM in MHCC97H cells. Results are presented as mean ± standard error of the mean, n = 3. ∗P < .05; ∗∗P < .01 by the Student t test. E, The cell apotosis by flow cytometry stably depleting HNRNPM in MHCC97H cells. Results are presented as mean ± standard error of the mean, n = 3. ∗P < .05; ∗∗P < .01 by the Student t test. F, Cell cycle detected by flow cytometry when depleting HNRNPM in MHCC97H cells. G, Colony formation assay when depleting HNRNPM in MHCC97H cells. Results are presented as mean ± standard error of the mean, n = 3. ∗P < .05; ∗∗P < .01 by the Student t test. H, Cell migration assay when depleting HNRNPM in MHCC97H cells. Results are presented as mean ± standard error of the mean, n = 3. ∗∗∗P < .001 by the Student t test. I, Cell invasion assays when depleting HNRNPM in MHCC97H cells. J-K, The in vivo effects in BALB/c nude mice (n = 6 per group) when overexpressed and depleted HNRNPM. Results are presented as mean ± standard error of the mean, n = 6. ∗P < .05; ∗∗P < .01 by the Student t test. L-M, The number of liver metastasis in BALB/c nude mice when overexpressed and depleted HNRNPM. Results are presented as mean ± standard error of the mean, n = 6. ∗P < .05; ∗∗P < .01 by the Student t test. N, The CSC frequency was determined from a limiting dilution assay performed with HCC cells from the third transplant recipient mice. The ELDA web tool was used to calculate the frequency of CSCs.