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. 2022 Feb 12;13(5):1413–1447. doi: 10.1016/j.jcmgh.2022.02.006

Figure 9.

Figure 9

DNA methylation controls MBD2-mediated FZD3 transcription.A, The shematic diagram of HNRNPM domains. B, The specific binding site for MBD2 with HNRNPM by CLIP assay. C, The luciferase assay for FZD3 transcription activity when overexpressing MBD2a or MBD2a and MBD2c. Data were from three independent experiments. ∗P < .05. P values were calculated using 1-way analysis of variance and Dunnett’s multiple comparison test. D-H, qPCR analysis of MBD2a, MBD2c, FZD3, β-catenin, and Snail1 mRNA transcripts in MHCC97H cells stably expressing NC, shRNAs targeting HDAC1, HDAC2, RBBP7, or MTA2. Immunoblot analysis showed the knockdown efficiency of shRNAs targeting HDAC1, HDAC2, RBBP7, or MTA2 in MHCC97H cells. Data were from 3 independent experiments. ∗P < .05. P values were calculated using 1-way analysis of variance and Dunnett’s multiple comparison test. I-J, β-catenin promotes the expression of OCT4 (I) and SOX2 (J) by binding its promoter. Data were from 3 independent experiments. ∗P < .05. P values were calculated using 1-way analysis of variance and Dunnett’s multiple comparison test.