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. 2022 Mar 21;13:1509. doi: 10.1038/s41467-022-29007-y

Fig. 4. Muropeptide composition of B. bacteriovorus HD100.

Fig. 4

a–d Muropeptide elution profiles obtained by HPLC. Peptidoglycan sacculi were isolated from attack-phase B. bacteriovorus cells. a Wild-type (WT) HD100, b ∆bd1075, c ∆bd1075 (pbd1075HD100)—bd1075HD100 expressed in ∆bd1075, and d ∆bd1075 (pEV)—empty vector control in ∆bd1075. Sacculi were digested by cellosyl and the resulting muropeptides were reduced with sodium borohydride and analyzed by HPLC. Representative chromatograms of 2 biological repeats are shown. e HPLC muropeptide elution profiles of ∆bd1075 sacculi treated with either purified Bd1075HD100 enzyme (above) or buffer control (below). Data are from 1 biological repeat. f Structures of the seven main muropeptide fractions. Numbers correspond to those above peaks in a–e and were assigned based on the retention times of corresponding known E. coli muropeptides. G: N-acetylglucosamine, M: N-acetylmuramitol, L-Ala: L-alanine, D-iGlu: D-glutamic acid, meso-Dap: meso-diaminopimelic acid, D-Ala: D-alanine. Minor peaks are annotated in Supplementary Fig. 17 (for a–d) and Supplementary Fig. 16a (for e) and were assigned by mass spectrometry analysis (Supplementary Table 2).