TABLE 1.
Oligonucleotides used in site-directed mutagenesis experiments and in DNA sequencing
| Procedure and oligonucleotide | Sequence (5′-3′)a | Codon change and nucleotide positionb |
|---|---|---|
| Mutagenesis | ||
| Ile86→Val | GGCGTCAACACGGGATAAT | ATT→GTT |
| Val184→Ala | ATTGCGGCAGGCATCGTGG | GTA→GCC |
| Met69→Leu | TAAAAGTGCTCAGCATTGGAAAAC | ATG→CTG |
| Asn276→Asp | TCTGTCTATCTCGTTCATCC | AAT→GAT |
| Arg244→Cys | ATGATACCGCAAGACCC | CGC→TGC |
| Glu104→Lys | GTGAGTATTTAACCAAGTC | GAG→AAA |
| Gly238→Ser | CACGCTCACTGGCTCCAGATTTAT | GGT→AGT |
| Arg164→Ser | GTTCCCAAGAATCAAGGC | CGT→TCT |
| Sequencing | ||
| f-TEM2F | GTATGAGTATTCAACATTTCCGTGTCG | 205-231 |
| f-TEM2R | ACCAATGCTTAATCAGTGAGGCA | 1064-1042 |
| f-TEMi | ACTGTCATGCCATCCGTAAGA | 556-536 |
| f-TEM2i | CTGCGGCCAACTTACTTCTGACAA | 598-621 |
a
Altered bases are underlined.
b
Nucleotide positions are according to Sutcliffe (27).