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Representative immunohistochemistry slides of sarcoma tumors in (A) Rag2−/−, (B) Rag2+/− mice stained with F4/80 (macrophage marker). The slides were analyzed and the percentage area positive for F4/80 was found not to be significantly different between the two genotypes (p = 0.62, unpaired t-test) (C). Flow cytometry confirmed significantly higher T cell infiltrate in tumors from Rag2+/− compared to Rag2−/− mice (p = 0.01, unpaired t-test) (D).
