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Conditions for polar gradient (mobile phases, step 49)
| Time (min) | Flow rate (mL/min) | Mobile phase A (%) |
Mobile phase B (%) |
|
|---|---|---|---|---|
| 1 | 0.00 | 0.2 | 25.0 | 75.0 |
| 2 | 2.00 | 0.2 | 25.0 | 75.0 |
| 3 | 14.00 | 0.2 | 75.0 | 25.0 |
| 4 | 18.00 | 0.2 | 75.0 | 25.0 |
| 5 | 19.0 | 0.2 | 25.0 | 75.0 |
| 6 | 23.0 | 0.2 | 25.0 | 75.0 |
