Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Conditions for semi-polar gradient (mobile phases, step 51)
| Time (min) | Flow rate (mL/min) | Mobile phase A (%) |
Mobile phase B (%) |
|
|---|---|---|---|---|
| 1 | 0.00 | 0.3 | 100.0 | 0.0 |
| 2 | 22.00 | 0.3 | 72.0 | 28.0 |
| 3 | 36.00 | 0.3 | 0.0 | 100.0 |
| 4 | 38.00 | 0.3 | 0.0 | 100.0 |
| 5 | 38.50 | 0.3 | 100.0 | 0.0 |
| 6 | 40.00 | 0.3 | 100.0 | 0.0 |
