Table 4. Results of the Solid-Phase Synthesis of PB/PS/PO Chimeric ODNs.
| isolated yieldb |
m/z |
||||
|---|---|---|---|---|---|
| entry | abbreviation of ODNs | producta | (%) | calcd | found |
| 1 | d(CPSAPBGPOT) (19) | 592.6236 | 592.6209 | ||
| 2 | d(CPOAPBGPST) (20) | 592.6236 | 592.6217 | ||
| 3 | d(CPSAPSGPSTPSCPBAPBGPBTPBCPOAPOGPOT) (21) | 6 | 615.7786 | 615.7767 | |
| 4 | PB/PS/PO | d(GPBCPSAPBTPOTPOGPOGPOTPSAPBTPSTPBC) (22) | 19 | 614.1138 | 614.1136 |
| 5 | PB/PS | d(GPBCPSAPBTPSTPSGPBGPBTPSAPBTPSTPBC) (23) | 5 | 618.6194 | 618.6175 |
| 6 | PB/PO | d(GPBCPBAPBTPOTPOGPOGPOTPBAPBTPBTPBC) (24) | 19 | 604.9781 | 604.9774 |
| 7 | PB/PS/PO-gapmer | GPBCPBAPB d(TPSTPOGPOGPOTPSAPB)UPBUPBC (25) | 13 | 635.9695 | 635.9670 |
a
Subscript PB = boranophosphate, PS = phosphorothioate, PO = phosphate, underline = 2′-OMe modification.
b
Determined by UV absorbance 260 nm.