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. Author manuscript; available in PMC: 2022 Mar 22.
Published in final edited form as: J Am Chem Soc. 2022 Feb 10;144(7):3174–3181. doi: 10.1021/jacs.1c12750

Figure 5.

Figure 5.

Hairpin-like design increases gene silencing durability of siRNA-SNAs. (A) Full gene silencing activity of HER2-targeting siRNA-SNAs. SK-OV-3 cells were transfected with 100 nM siRNA equivalent SNAs. Relative gene expression was measured 48 h after SNA administration by RT-qPCR, normalized to transfection agent-only treatment. Error bars are SD of 3 experimental replicates. (B) Knockdown potency of HER2-targeting siRNA-SNAs 2 days after SNA administration. SK-OV-3 cells were transfected with a range of SNA concentrations. Protein expression was measured using an in-cell Western assay, normalized to transfection agent-only treatment. Dotted lines are 95% CI. Error bars are SD of 3 biological replicates. (C) Knockdown potency of HER2-targeting siRNA-SNAs 4 weeks after SNA administration. Dotted lines are 95% CI. Error bars are SD of 3 biological replicates. (D) IC50 of HER2-targeting siRNA-SNAs at 2 days and 4 weeks after SNA administration, based on protein expression curves in (B) and (C). Error bars are SD of 3 biological replicates (ns, P > 0.05, *P ≤ 0.05).