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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Clin Immunol. 2021 Jan 8;41(3):639–657. doi: 10.1007/s10875-020-00930-3

Figure 2. Protein expression of GATA2 alleles in an overexpression system.

Figure 2.

HEK293T cells were transfected with an empty plasmid (EV), a construct encoding GATA2 wild-type (WT)-DDK-tagged or mutated alleles corresponding to isoform 1 of GATA2 (p.R330*, p.M388T, p.R396W, p.R396Q, p.R398Q, p.R398W, c.599dup, c.915_916del, c.1023del, c.1035_1036insTCTGGCC, c.1099dup, p.V382Gfs*23, p.N381_V382ins41 and p. S340_N381del) for 48 hours. GATA2 protein levels were evaluated by western blotting with antibodies against GATA2 and against the DDK tag. GAPDH was used as a loading control. NT indicates non-transfected cells. The results shown are representative of three independent assays.