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. Author manuscript; available in PMC: 2023 Feb 16.
Published in final edited form as: J Am Chem Soc. 2022 Feb 2;144(6):2667–2678. doi: 10.1021/jacs.1c11341

Figure 1.

Figure 1.

Plot showing temperature-dependent changes in average charge (Zavg) of GroEL14 and GroEL14–ATPn (n = 1–9) (adapted from ref 25). Solution conditions are 1 μM GroEL, 200 mM ammonium acetate, and 125 μM ATP. Note that Zavg for n = 1–4 are similar, but larger changes in Zavg are observed for n = 5–9. At T >24 °C the ion abundances for n = 6–9 are below the detection threshold. Also, the abundance of n = 5 begins to decline at T >24 °C. This behavior is very different from that obtained by using ethylenediammonium diacetate buffer (vide infra).