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. Author manuscript; available in PMC: 2023 Feb 16.
Published in final edited form as: J Am Chem Soc. 2022 Feb 2;144(6):2667–2678. doi: 10.1021/jacs.1c11341

Table 1.

Effects Mg2+ and ATP Concentrations on ATP Binding and Stoichiometry of GroEL–ES Complex at 24 °C in 200 mM EDDA

solution “X” GroEL (nM) Mg2+ (μM) ATP (μM) GroES (μM) ATP/GroEL Mg2+/GroEL GroELx–GroESy–ATPn
A 500 1000 10 20 2000 GroEL14–ATP1–4
B 500 1000 25 50 2000 GroEL14–ATP1–14
C 500 1000 50 100 2000 GroEL14–ATP3–14
D 260 225 110 12 420 850 GroEL14–GroES14–ATP14
E 120 25 50 12 420 200 GroEL14–GroES7–8–ATP8
F 600 500 50 12 80 850 GroEL14–GroES7–8–ATP8
G 600 25 50 12 80 40 GroEL14–GroES0–2–ATP0–2
H 500 12 not detected