Fig. 3.

Nlrp12 suppression of uveitis involves regulation of BM-derived myeloid cellular responses. A–C Leukocytic infiltrate in the eyes of WT and Nlrp12^−/− mice 21 d post-immunization was evaluated by flow cytometry. Analysis was performed on live singlets expressing the pan-leukocyte marker CD45. A Representative contour plot and summary statistics depicting proportion and number of CD11b⁺ cells of gated CD45⁺ cells. B Contour plot and summary statistics of gated CD11b⁺ cells further distinguished as monocyte–macrophages (being CD45^high, CD11c^lo, F4/80^high) vs. neutrophils (CD45^high, CD11c^lo, F4/80^loGR-1^high). C Expression of activation markers within the gated monocyte/macrophage population. A–C Data are mean ± SEM of pooled eyes (5 mice/group) and combined across 3 independent experiments; *p < 0.05 by one-tailed Mann–Whitney. D Bone-marrow (BM)-chimeric mice were generated by transplantation of WT or Nlrp12^−/− BM into irradiated WT or Nlrp12^−/− recipients. Chimeras were immunized 8 weeks later, and eyes were evaluated for uveitis by histopathology 28 day post-immunization. Data are mean ± SEM (n = 9–10 mice/group combined from 2 experiments), *p < 0.05 by one-tailed Mann–Whitney. E Expression levels of cytokine/chemokine genes in neuroretinas of IRBP_1–20-immunized WT and Nlrp12^−/− mice (14 day post-immunization, i.e., prior to clinical onset of uveitis) were evaluated by multiplex RT-qPCR. Colors in heatmap indicate the expression level of transcripts relative to control adjuvant-injected WT mice (n = 5 retinas pooled/experimental group)