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. 2022 Mar 22;11:15. doi: 10.1186/s40164-022-00270-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Analysis of CD19 and CD22 cell-surface expression in three patients at pretreatment and relapsed stage. BMMCs from patients before CAR-T cells infusion and relapsed after immunotherapy were obtained, stained with CD45, CD34, CD10, CD33, CD19 and CD22 and analyzed by flow cytometry. Leukemia blasts were gated via CD45/SSC two parameters graph and subgated in CD34 or CD10, followed by analysis of surface expression of CD19 and CD22 on gated cells. Red dots represent leukemia blasts. Black dots represent normal cells. a, b and e. Flow cytometry analysis of BMMCs samples from 3 patients at pretreatment and relapse stage, including patient 4 (a), patient 3 (b) and patient 2 (e). c. CD19 expression in patient 3 by histogram analysis at pretreatment and relapse stage indicated loss of CD19 antigen during treatment. d Histogram analysis of CD22 on BMMCs samples from patient 3 before CAR-T cells infusion and relapse stage showed CD22 downregulation