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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Neurobiol Dis. 2021 Aug 8;158:105470. doi: 10.1016/j.nbd.2021.105470

Figure 3.

Figure 3.

Neuronal SOD2 deficiency induces a metabolic switch toward aerobic glycolysis. (A) Representative trace of a glycolytic stress test performed on primary cortical cells derived from pnd 2-3 nSOD2 KO and WT littermates (n=10 wells/animal, representing 6 WT, 10 KO, across 3 experiments). (B-D) Extracellular acidification rates measured key parameters of glycolytic flux, including (B) glycolysis (p=0.0088), (C) glycolytic capacity (p=0.0047), and (D) glycolytic reserve (p=0.0265). (E) Glucose levels were measured in 2 month old mice and found to be significantly decreased in plasma (unpaired t test, n=9-12, p<0.0001) but increased in cortical tissue (unpaired t test, n=11-12, p=0.0104). (F) Gene expression analysis of glucose transporters in cortical tissue of 2 m old nSOD2 mice (unpaired t test, n=12/group).