FIG 4.

Structure alignment and phylogenetic analysis of dmpKLMNOP. (A) Amino acid sequence identity analysis between the putative THF hydroxylase gene cluster dmpKLMNOP in C. metallidurans ZM02 and other reported THF/phenol-degrading gene clusters. The blue box represents the reported THF/14D-degrading gene clusters, and the purple box represents the reported phenol-degrading gene clusters. The arrows indicate the transcript size and direction of the transcription of each gene, and genes with the same filled color are isoenzymes. The numbers under the genes represent the amino acid sequence identity of each component of MOs with the corresponding component in strain ZM02. (B) Unrooted phylogenetic tree generated based on amino acid sequences of the large subunit of SDIMOs. SDIMOs divided into the same group are represented by the same color. The strains marked with a gray triangle can utilize THF as a sole carbon source. Group 1, toluene/benzene MOs; group 2, phenol MOs; group 3, methane/butane MOs; group 4, alkene MOs; group 5, THF MOs; group 6, propane MOs.