FIG 7.

Detection of THF catalytic activity in cell extracts of strain E. coli BL21(DE3) containing plasmid pET28a-dmpKLMNOP or empty vector pET28a. The reaction was initiated by the addition of 0.2 mM THF, and the spectra were recorded every minute after the addition of THF for 10 min. The arrows indicate the directions of spectral changes. (A) The reaction mixtures of the control group contained cell extracts of E. coli BL21(DE3) containing plasmid pET28a-dmpKLMNOP, NADH, and Fe²⁺. (B) The reaction mixtures of the control group contained cell extracts of E. coli BL21(DE3) containing plasmid pET28a-dmpKLMNOP, NADPH, and Fe²⁺. (C) The reaction mixtures of the control group contained cell extracts of E. coli BL21(DE3) containing empty vector pET28a, NADPH, THF, and Fe²⁺. (D) The reaction mixtures contained cell extracts of E. coli BL21(DE3) containing plasmid pET28a-dmpKLMNOP, NADH, THF, and Fe²⁺. (E) The reaction mixtures contained cell extracts of E. coli BL21(DE3) containing plasmid pET28a-dmpKLMNOP, NADPH, THF, and Fe²⁺.