TABLE 1.
Growth of the wild-type strains and gene knockout strains on different substratesa
| Substrate | Strain (mean growth [g dry weight] ± SD) |
|||||
|---|---|---|---|---|---|---|
| ΔdmpL | ΔdmpN | ΔdmpP | ΔdmpR | ZM02 | JMP134 | |
| Tetrahydrofuran | NG | NG | NG | NG | 0.30 ± 0.02 | NG |
| 2-Hydroxytetrahydrofuran | 0.39 ± 0.03 | 0.40 ± 0.00 | 0.37 ± 0.03 | 0.52 ± 0.00 | 0.42 ± 0.01 | 0.37 ± 0.03 |
| γ-Butyrolactone | 0.59 ± 0.01 | 0.66 ± 0.02 | 0.58 ± 0.04 | 0.63 ± 0.01 | 0.65 ± 0.03 | 0.18 ± 0.00 |
| Phenol | NG | NG | NG | NG | 0.64 ± 0.01 | 0.79 ± 0.03 |
| Catechol | 0.58 ± 0.11 | 0.79 ± 0.03 | 0.50 ± 0.09 | 0.72 ± 0.04 | 0.66 ± 0.05 | 0.32 ± 0.01 |
All of the strains were cultured in AMS medium containing 5 mM of each substrate at an initial OD600 of 0.05 for 5 days at 30°C with 200 rpm. NG, no growth.