TABLE 2.
Crude enzyme activity of different THF hydroxylase expression strains with THF
| Reaction conditions | Mean enzyme activity (μmol min−1 mg−1) ± SDa |
||
|---|---|---|---|
| C. pinatubonensis JMP134(pBBR-dmpKLMNOP) | C. pinatubonensis JMP134(pHGE-dmpKLMNOP) | E. coli BL21(DE3)(pET28a-dmpKLMNOP) | |
| Cell extract+NADH+THF | ND | ND | 0.29 ± 0.11 |
| Cell extract+NADH+Fe2+ | ND | ND | ND |
| Cell extract+NADH+Fe2++THF | ND | ND | 0.53 ± 0.07 |
| Cell extract+NADPH+THF | ND | ND | 1.04 ± 0.20 |
| Cell extract+NADPH+Fe2+ | ND | ND | ND |
| Cell extract+NADPH+Fe2++THF | ND | ND | 1.38 ± 0.24 |
a
ND, not detectable. The numbers indicate the THF-stimulated NAD(P)H oxidation rates.