TABLE 3.
Crude enzyme activity of different THF hydroxylase expression strains with phenol
| Reaction conditions | Mean enzyme activity (μmol min−1 mg−1) ± SDa |
||
|---|---|---|---|
| C. pinatubonensis JMP134(pBBR-dmpKLMNOP) | C. pinatubonensis JMP134(pHGE-dmpKLMNOP) | E. coli BL21(DE3)(pET28a-dmpKLMNOP) | |
| Cell extract+NADH+phenol | ND | ND | 0.82 ± 0.09 |
| Cell extract+NADH+Fe2+ | ND | ND | ND |
| Cell extract+NADH+Fe2++phenol | ND | ND | 1.19 ± 0.14 |
| Cell extract+NADPH+phenol | ND | ND | 1.54 ± 0.23 |
| Cell extract+NADPH+Fe2+ | ND | ND | ND |
| Cell extract+NADPH+Fe2++phenol | ND | ND | 1.78 ± 0.37 |
a
ND, not detectable. The numbers indicate the phenol-stimulated NAD(P)H oxidation rates.