TABLE 4.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| C. metallidurans ZM02 | Wild type, THF-degrading strain; G-, Tcs | This study |
| C. pinatubonensis JMP134 | Wild type, phenol-degrading strain, used for gene expression; G-, Kms | 60 |
| S. melonis TY | Wild type, phenol-degrading strain | 61 |
| A. faecalis JQ135 | Wild type, phenol-degrading strain | 62 |
| E. coli | ||
| DH5α | supE44 lacU169(80dlacZΔM15) hsdR17 recA1 endA1 gyrA96 Δthi relA1 | Tsingke |
| BL21(DE3) | Chaperonin gene groELS integrated in chromosome | 63 |
| ΔdmpL | ZM02 mutant with dmpL gene replaced by Tc resistance gene | This study |
| ΔdmpN | ZM02 mutant with dmpN gene replaced by Tc resistance gene | This study |
| ΔdmpP | ZM02 mutant with dmpP gene replaced by Tc resistance gene | This study |
| ΔdmpR | ZM02 mutant with dmpR gene replaced by Tc resistance gene | This study |
| JMP134-pBBR | JMP134 transformed with pBBR1MCS2; Kmr | This study |
| JMP134-pBBR-dmpKLMNOPR | JMP134 transformed with pBBR-dmpKLMNOPR; Kmr | This study |
| JMP134-pBBR-dmpKLMNOP | JMP134 transformed with pBBR-dmpKLMNOP; Kmr | This study |
| JMP134-pBBR-dmpKLMNO | JMP134 transformed with pBBR-dmpKLMNO; Kmr | This study |
| JMP134-pBBR-dmpLMNOP | JMP134 transformed with pBBR-dmpLMNOP; Kmr | This study |
| JMP134-pBBR-dmpKLMN | JMP134 transformed with pBBR-dmpKLMN; Kmr | This study |
| JMP134-pHGE | JMP134 transformed with pHGE; Kmr | This study |
| JMP134-pHGE-dmpKLMNOP | JMP134 transformed with pHGE-dmpKLMNOP; Kmr | This study |
| BL21(DE3)-pET28a | BL21(DE3) transformed with pET28a; Kmr | This study |
| BL21(DE3)-pET28a-dmpKLMNOP | BL21(DE3) transformed with pET28a-dmpKLMNOP; Kmr | This study |
| Plasmids | ||
| pEX18Tc | Gene knockout vector, oriT+, sacB+; Tcr | 64 |
| pEX18Tc-dmpL | dmpL gene knockout vector; Tcr | This study |
| pEX18Tc-dmpN | dmpN gene knockout vector; Tcr | This study |
| pEX18Tc-dmpP | dmpP gene knockout vector; Tcr | This study |
| pEX18Tc-dmpR | dmpR gene knockout vector; Tcr | This study |
| pBBR1MCS2 | Gene expression vector; Kmr | 64 |
| pBBR-dmpKLMNOPR | Vector for expression of dmpKLMNOPR; Kmr | This study |
| pBBR-dmpKLMNOP | Vector for expression of dmpKLMNOP; Kmr | This study |
| pBBR-dmpKLMNO | Vector for expression of dmpKLMNO; Kmr | This study |
| pBBR-dmpKLMN | Vector for expression of dmpKLMN; Kmr | This study |
| pBBR-dmpLMNOP | Vector for expression of dmpLMNOP; Kmr | This study |
| pHGE | Ptac, IPTG-inducible expression vector; Kmr | 65 |
| pHGE-dmpKLMNOP | Vector for inducible expression of dmpKLMNOP; Kmr | This study |
| pET28a | Gene expression vector; Kmr | Novagen |
| pET28a-dmpKLMNOP | Vector for inducible expression of dmpKLMNOP; Kmr | This study |
a
Tcr, tetracycline resistance; Kmr, kanamycin resistance; Kms, kanamycin sensitivity; G-, gram-negative bacterium.