TABLE 5.
Primers used in this study
| Primer | Sequence (5′–3′)a | Purpose |
|---|---|---|
| dmpL-UF | CGGTACCCGGGGATCCCGTAGCCAAGCTGCACCCA | Construct dmpL gene knockout vector |
| dmpL-UR | ATAAACAAATAGGGGTTCCGCGGGTGGTTGTCTCCTCGGATGT | |
| dmpL-DF | AGCCGGGCCACCTCGACCTGACGGAGACAACCATGAGCGAC | |
| dmpL-DR | GGCCAGTGCCAAGCTTGAACGTCACCGTGGCCATGT | |
| dmpN-UF | CGGTACCCGGGGATCCTCGCTCTTGCGCACGAAGAAAC | Construct dmpN gene knockout vector |
| dmpN-UR | ATAAACAAATAGGGGTTCCGCGACAAGGAGACCAGATCATGCCAG | |
| dmpN-DF | AGCCGGGCCACCTCGACCTGAGGTTTGTCTCCTGTCTTGGGGTT | |
| dmpN-DR | GGCCAGTGCCAAGCTTATTTCGTGGACAAGCGAGGGC | |
| dmpP-UF | CGGTACCCGGGGATCCCTGGTCCTGCAACGCTAAAGC | Construct dmpP gene knockout vector |
| dmpP-UR | ATAAACAAATAGGGGTTCCGCGAGTATTTCCACCACAAAGGAGACA | |
| dmpP-DF | AGCCGGGCCACCTCGACCTGAGGCAACCTCCTGCAGCAAGT | |
| dmpP-DR | GGCCAGTGCCAAGCTTTTCGAGCAGAACGGTGGGGC | |
| dmpR-UF | CGGTACCCGGGGATCCAGGCGGTAATCGACGCGTTG | Construct dmpR gene knockout vector |
| dmpR-UR | ATAAACAAATAGGGGTTCCGCGCCTTGTCACTCCAGATTCCGGC | |
| dmpR-DF | AGCCGGGCCACCTCGACCTGAGGTTGGGACAGTTGGCCCAG | |
| dmpR-DR | GGCCAGTGCCAAGCTTTGTGCGCGGACGGATTACCG | |
| Tc-F | CGCGGAACCCCTATTTGTTTAT | Amplify Tc gene |
| Tc-R | TCAGGTCGAGGTGGCCCGGC | |
| pEX18Tc-F | ACTCATTAGGCACCCCAGGC | Verify gene knockout vector |
| pEX18Tc-R | CAAGCTCGCCATTCGCCATT | |
| ΔdmpL-UF | TTGACCACTTCCAGGCCCAGC | Verify dmpL gene knockout mutant |
| ΔdmpL-UR | GGAAGCAGCCCAGTAGTAGGT | |
| ΔdmpL-DF | ATATCGCCGACATCACCGATG | |
| ΔdmpL-DR | CGCTCGCATTCGGTTTCCTTG | |
| ΔdmpN-UF | TCTGCCGCCGACAGGAACTT | Verify dmpN gene knockout mutant |
| ΔdmpN-UR | AGTGCGGCGACGATAGTCATG | |
| ΔdmpN-DF | CGTCCTGTGGATTCTCTACGCC | |
| ΔdmpN-DR | AGACCTTCGCGCATATCGCG | |
| ΔdmpP-UF | TGTTCCTGTCCCTGCGGCAC | Verify dmpP gene knockout mutant |
| ΔdmpP-UR | GCTGACTGGGTTGAAGGCTCT | |
| ΔdmpP-DF | GACTGTTGGGCGCCATCTCC | |
| ΔdmpP-DR | CTACAACGGGGACATGGCCAC | |
| ΔdmpR-UF | CTGCCAGGCCATGTCTTCCAT | Verify dmpR gene knockout mutant |
| ΔdmpR-UR | AGGGCGTGCAAGATTCCGAA | |
| ΔdmpR-DF | ATGCAGGAGTCGCATAAGGG | |
| ΔdmpR-DR | CGACATGGGTCAGCACCAGAT | |
| pBBR-dmpKR-F | CGGTATCGATAAGCTCTACTTCTTCAACCGATACGCCAGC | Design pBBR-dmpKLMNOPR expression vector |
| pBBR-dmpKR-R | CGGGCTGCAGGAATTTCAGAGTGAGCGGAACAGCG | |
| pBBR-dmpKP-F | CGGTATCGATAAGCTATGAGATATCCCCCTCAGGCAC | Design pBBR-dmpKLMNOP expression vector |
| pBBR-dmpKP-R | CGGGCTGCAGGAATTTCAGAGTGAGCGGAACAGCG | |
| pBBR-dmpKO-F | CGGTATCGATAAGCTATGAGATATCCCCCTCAGGCAC | Design pBBR-dmpKLMNO expression vector |
| pBBR-dmpKO-R | CGGGCTGCAGGAATTTCAGTTGGCCGAGCCGGC | |
| pBBR-dmpKN-F | CGGTATCGATAAGCTATGAGATATCCCCCTCAGGCAC | Design pBBR-dmpKLMN expression vector |
| pBBR-dmpKN-R | CGGGCTGCAGGAATTTCACGAACGGTTGTTGCCTTG | |
| pBBR-dmpLP-F | CGGTATCGATAAGCTATGCAAATCGATATCCGGACTG | Design pBBR-dmpLMNOP expression vector |
| pBBR-dmpLP-R | CGGGCTGCAGGAATTTCAGAGTGAGCGGAACAGCG | |
| pBBR-F | AGCGCGCAATTAACCCTCACT | Verify gene expression vector |
| pBBR-R | ACTATAGGGCGAATTGGAGCTCC | |
| pHGE-dmpKP-F | CACAGGAGAGAATTCATGACCACGCCCATCGTCCA | Design pHGE-dmpKLMNOP expression vector |
| pHGE-dmpKP-R | CAAAACAGCCAAGCTTTCAGAGCGAGCGGAACAGC | |
| pHGE-F | ATGAGCCGTGTTTTCTGGACGAT | Verify gene expression vector |
| pHGE-R | ATGCCTGGCAGTTCCCTACTCT | |
| pET28-dmpKP-F | AGGAGATATACCATGAGATATCCCCCTCAGGCAC | Design pET28a-dmpKLMNOP expression vector |
| pET28-dmpKP-R | AGCCGGATCTCAGTGGTGGTGGTGGTGGTGTCAGAGTGAGCGGAACAGCG | |
| pET28a-F | AGCCCAGTAGTAGGTTGAGGCC | Verify gene expression vector |
| pET28a-R | AGCAAAAAACCCCTCAAGACCCG | |
| dmpL-qPCR-F | GGAGAAGAACCTTGATTTC | RT-PCR |
| dmpL-qPCR-R | TCCATCGTGTGATACATC | |
| dmpN-qPCR-F | GAAGTGGTCAAGTTCATG | RT-PCR |
| dmpN-qPCR-R | TGTAGTCCATCATCATCG | |
| dmpP-qPCR-F | CCGATGACGAACTGTATT | RT-PCR |
| dmpP-qPCR-R | GAAGTCGTAGCCGAAATG | |
| dmpR-qPCR-F | CTTCTATGGCGAGTTTCT | RT-PCR |
| dmpR-qPCR-R | CATTCGGTTTCCTTGTAG | |
| catA-qPCR-F | GGCAACTACTCGTACTTC | RT-PCR |
| catA-qPCR-R | GAGACGAAGAAATGGATATG | |
| 16S rRNA-qPCR-F | CCTACGGGAGGCAGCAG | RT-PCR, 16S rRNA |
| 16S rRNA-qPCR-R | ATTACCGCGGCTGCTGG |
a
Underlined nucleic acid sequences represent homologous sequences derived from plasmids.