| PcAA14A |
ThermoPETase |
1.3 |
PET granules (3 g/L)
incubated with 8.3 mgEnzyme/gPET in glycine-NaOH
buffer (pH 9) for 5 days |
(137) |
| RolA |
IsPETase |
1.2 (by weight loss) |
Preincubation
with RolA for 3 h, then degradation
of PET fiber waste and high-crystallinity PET powder (15 g/L) at pH
8, 30 °C with 1.3 gEnzyme/gPET for 5 days |
(136) |
| 1.5 (by HPLC) |
| HGFI |
IsPETase |
1.3 (by weight loss) |
| 1.6 (by HPLC) |
| Zwitterionic polypeptide (EK) |
IsPETase |
11 |
Degradation of amorphous
Goodfellow PET film and high-crystallinity
(45.2%) beverage bottle with 200 nM enzyme in glycine-NaOH-buffer
at pH 9 and 30–40 °C for up to 6 days |
(138) |
| CBM |
A cutinase from T. fusca
|
1.4–1.5 (only affinity) |
Incubated with PET fiber and
50 μM enzyme in 50 mM Tris-buffer
(pH 8) at 50 °C for 24 h |
(104) |
| ChBD |
LCCICCG
|
1.2 |
With 0.6 g/L amorphous Goodfellow PET film, post-consumer
waste PET (16% crystallinity), or high crystallinity (40%) PET at
65 °C for 12 h |
(103) |
| CBM |
LCCICCG
|
1.3 |
| CBM |
ThC_Cut1 |
1.4 |
With amorphous Goodfellow PET film and 25 mM enzyme
in 100 mM potassium phosphate buffer (pH 7) at 50 °C |
(100) |
| PBM |
ThC_Cut1 |
3.8 |
