FIG 2.

Knockdown of the spds gene decreases the content of GAs by decreasing ROS production. (A) ROS levels determined by DCHF-DA staining in the WT, Control si, two spds knockdown strains, and strains supplied with 1 mM spermidine (Spd) from 5-day-old plate mycelia. Bar = 50 μm. (B) Relative fluorescence intensities from panel A. (C) H₂O₂ contents in the WT, Control si, two spds knockdown strains, and strains supplied with 1 mM Spd from 5-day-old plate mycelia. (D) GA contents in the WT, Control si, two spds knockdown strains, and strains supplied with 5 mM H₂O₂ from 7-day-old liquid mycelia. (E) GA contents in the WT, Control si, two spds knockdown strains, and strains supplied with 1 mM Spd and treated with 2 mM ascorbic acid (Vc) or 1 mM N-acetyl cysteine (NAc) from 7-day-old liquid mycelia. The values presented are the means ± SD of data from three independent experiments. Different letters indicate significant differences between the lines (P < 0.05, according to Duncan’s multiple-range test).