FIG 3.

Knockdown of the spds gene decreases mitochondrial ROS (mtROS) production. (A) Mitochondrial ROS levels determined by DCHF-DA and MitoTracker Red double staining in the WT, Control si, and two spds knockdown strains from 5-day-old plate mycelia. Bar = 50 μm. (B) Relative fluorescence intensities from panel A. The values presented are the means ± SD of data from three independent experiments. Different letters indicate significant differences between the lines (P < 0.05, according to Duncan’s multiple-range test).