FIG 5.

Loss of activity of mitochondrial complexes I and II leads to decreases in mitochondrial ROS (mtROS) production and GA contents. (A) Relative activities of mitochondrial complexes I, II, III, and IV in the WT, Control si, and two complex I (CIA30) and complex II (SDHB) coknockdown strains from 5-day-old plate mycelia. (B) Mitochondrial ROS levels determined by DCHF-DA and MitoTracker Red double staining in the WT, Control si, and two CIA30 and SDHB coknockdown strains from 5-day-old plate mycelia. Bar = 50 μm. (C) Relative fluorescence intensities from panel B. (D) H₂O₂ contents in the WT, Control si, and two CIA30 and SDHB coknockdown mutants from 5-day-old plate mycelia. (E) GA contents in the WT, Control si, two CIA30 and SDHB coknockdown strains, and strains supplied with 5 mM H₂O₂ from 7-day-old liquid mycelia. The values presented are the means ± SD of data from three independent experiments. Different letters indicate significant differences between the lines (P < 0.05, according to Duncan’s multiple-range test).