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. 2022 Mar 22;88(6):e02037-21. doi: 10.1128/aem.02037-21

FIG 6.

FIG 6

Knockdown of the spds gene blocks the translation of complexes I and II. (A) Protein contents of complex I (anti-CIA30 [assembly factor NDUFAF1]) and complex II (anti-SDHB [succinate dehydrogenase subunit B]) determined by Western blotting in the WT, Control si, and two spds knockdown strains from 5-day-old plate mycelia. (B) Relative densities from panel A. (C) Transcription and translation of complex I and II subunits assessed by qRT-PCR in the WT, Control si, and two spds knockdown strains from 5-day-old plate mycelia. (D to I) Polyribosome profiles of the WT, Control si, and spds/eIF5A/dhs/dohh knockdown strains from 5-day-old plate mycelia. The values presented are the means ± SD of data from three independent experiments. Different letters indicate significant differences between the lines (P < 0.05, according to Duncan’s multiple-range test).