FIG 7.

Blockade of translation of complexes I and II after spds knockdown due to a decrease in eIF5A hypusination. (A) eIF5A protein contents and eIF5A hypusination levels determined by Western blotting in the WT, Control si, and two spds knockdown strains from 5-day-old plate mycelia. (B) Relative densities from panel A. (C) Transcription and translation of complex I and II subunits assessed by qRT-PCR in the WT, Control si, and eIF5A/dhs/dohh knockdown strains from 5-day-old plate mycelia. (D) Protein contents of complex I (CIA30) and complex II (SDHB) determined by Western blotting in the WT, Control si, and eIF5A/dhs/dohh knockdown strains from 5-day-old plate mycelia. (E) Relative densities from panel D. (F) Relative activities of mitochondrial complexes I, II, III, and IV in the WT, Control si, and eIF5A/dhs/dohh knockdown strains from 5-day-old plate mycelia. The values presented are the means ± SD of data from three independent experiments. Different letters indicate significant differences between the lines (P < 0.05, according to Duncan’s multiple-range test).