FIG 8.

Knockdown of the eIF5A gene or blockade of eIF5A hypusination leads to decreases in mitochondrial ROS production and GA contents. (A) Mitochondrial ROS levels determined by DCHF-DA and MitoTracker Red double staining in the WT, Control si, and eIF5A/dhs/dohh knockdown strains from 5-day-old plate mycelia. Bar = 50 μm. (B) Relative fluorescence intensities from panel A. (C) H₂O₂ contents in the WT, Control si, and eIF5A/dhs/dohh knockdown strains from 5-day-old plate mycelia. (D to F) GA contents in the WT, Control si, eIF5A/dhs/dohh knockdown strains, and strains supplied with 5 mM H₂O₂ from 7-day-old liquid mycelia. The values presented are the means ± SD of data from three independent experiments. Different letters indicate significant differences between the lines (P < 0.05, according to Duncan’s multiple-range test).