TABLE 1.
Primers used for the construction of gene knockdown strainsa
| Primer | Sequence (5′–3′) | Description |
|---|---|---|
| CIA30 si-F | GGTACCAAACCTCAGTCAACCCTCTT | Gl26075; CIA30 |
| CIA30 si-R | ACTAGTAGTCAATGCCCAGCTCATA | |
| RT-CIA30-F | TCCCATCACAACCGACCTA | |
| RT-CIA30-R | TGTTTCCGCCCAACAGAG | |
| SDHB si-F | ACTAGTTTGGAACCCAGATGAGCC | Gl15912; complex II succinate dehydrogenase subunit B |
| SDHB si-R | TCTAGATCCTGTCCTCCTGCGACT | |
| RT-SDHB-F | TGCGGACCCATGATTCTC | |
| RT-SDHB-R | ATCCTTGCTCGCGTTTGT | |
| eIF5A si-F | GGTACCGTCCCTGCAAGATCGTCG | Gl21362; eukaryotic translation initiation factor 5A |
| eIF5A si-R | ACTAGTGCCTGCTCCTCACCCATA | |
| RT-eIF5A-F | CGATGAGCAGCACAACCA | |
| RT-eIF5A-R | GCCCTTGATGACGACGTG | |
| DHS si-F | GGTACCCTGGGCGTACAAAGTGAA | Gl31472; deoxyhypusine synthase |
| DHS si-R | ACTAGTCAAAGGTGGCTGCTACAA | |
| RT-DHS-F | CCTCGGCTACACCTCCAA | |
| RT-DHS-R | CGATGCGGTTCATTCCAC | |
| DOHH si-F | GGTACCGGCATCTCCTTCCTCGTT | Gl23607; deoxyhypusine hydroxylase |
| DOHH si-R | ACTAGTGCCGCTTCATCCACTCCT | |
| RT-DOHH-F | ATCATCTCGAAGGTGCCTAT | |
| RT-DOHH-R | CGAGGACGGACTCCAGTA | |
a
The sequences with underline were restriction sites that were used for plasmid construction. Gl, Ganoderma lucidum.