TABLE 2.
Primers used for transcription and translation analyses of mitochondrial complex Ia
| Primer | Sequence (5′–3′) | Description |
|---|---|---|
| Gl23611-RT-F | ACCTTGTTGGTCGGGAGT | NDUFA9 |
| Gl23611-RT-R | TGTGGGATGCGTTGAGAT | |
| Gl26075-RT-F | TCCCATCACAACCGACCTA | CIA30 |
| Gl26075-RT-R | TGTTTCCGCCCAACAGAG | |
| Gl21014-RT-F | AGTGGAACGGTTTCAGCATC | NDUFS1 |
| Gl21014-RT-R | CAGGGATCTCCTTCGTATTGAC | |
| Gl16611-RT-F | GCCCAACAACTGTCGCTAA | NDUFV1 |
| Gl16611-RT-R | GGATGCTCATCTCCTCCTCTAC | |
| GL15140-RT-F | CCAACCTCCTTGAGATGC | NDUFV2 |
| GL15140-RT-R | GTCCTTGGTGGTTTGACC | |
| GL30051-RT-F | CGCCTTCACGCCGCATAT | NDUFS2 |
| GL30051-RT-R | GGTCCGCTCCTTCCAGATA | |
| Gl29889-RT-F | CAACCTTATTCATTTGCGTCTG | NDUFS3 |
| Gl29889-RT-R | CTTCGCCAATCTGCTCCC | |
| GL17543-RT-F | CTGGGCGTCGTGTTTCGT | NDUFS7 |
| GL17543-RT-R | GGTAGTAACCTCCTCCATTCG | |
| GL31498-RT-F | CTGTTTCTCGCTCCTTCG | NDUFS8 |
| GL31498-RT-R | CACTTGGACCATCCTTGTAG | |
| 18S-RT-F | TCGAGTTCTGACTGGGTTGT | 18S rRNA gene as a reference gene |
| 18S-RT-R | TCCGTTGCTGAAAGTTGTAT | |
a
NDUFA9, NADH:ubiquinone oxidoreductase alpha subunit 9; CIA30, complex I intermediate-associated protein 30; NDUFS1, NADH:ubiquinone oxidoreductase core subunit S1; NDUFV1, NADH:ubiquinone oxidoreductase core subunit V1.