TABLE 3.
Densitometry analysis of NPT Western blotting for cell and supernatant fractionsa
| Fraction | Construct | MeHg concn (μM) | AUC (a.u.) |
|
|---|---|---|---|---|
| Avg | Range | |||
| Pellet | pET9a | 0 | 6,162.7 | 6,137.2–6,188.2 |
| 2 | 4,860.7 | 3,621.9–6,099.4 | ||
| RN23B | 0 | 3,995.3 | 3,326.0–4,664.5 | |
| 2 | 3,628.9 | 3,021.2–4,236.5 | ||
| Supernatant | pET9a | 0 | 223.6 | 112.3–334.9 |
| 2 | 378.4 | 204.0–552.8 | ||
| RN23B | 0 | 50.3 | 24.1–76.4 | |
| 2 | 1,923.2 | 1,828.4–2,017.9 | ||
a
Protein content for each strain at each MeHg concentration was analyzed via Western blotting and quantified using ImageJ. The average AUC values for all replicates and ranges are reported. n = 2.