TABLE 2.
Phosphorylation of GCV and ACV in HCMV-infected MRC-5 cellsa
| Virus or cell | Anabolites (pmol/106 cells)b of:
|
|||||||
|---|---|---|---|---|---|---|---|---|
| GCV
|
ACV
|
|||||||
| MP | DP | TP | Total | MP | DP | TP | Total | |
| AD169 | 0.7 ± 0.05 | 7.4 ± 0.6 | 80 ± 4.6 | 88 | 6.0 ± 0.5 | 2.7 ± 0.8 | 35 ± 6.3 | 44 |
| XbaF 4-3-1 | 0.2 ± 0.04 | 1.9 ± 0.2 | 7.6 ± 0.5 | 9.6 | 1.3 ± 0.1 | 0.7 ± 0.1 | 6.2 ± 1.1 | 8.2 |
| Mock-infected cells | 0.02 ± 0.01 | 0.2 ± 0.05 | 1.4 ± 0.1 | 1.6 | 0.2 ± 0.06 | 0.3 ± 0.03 | 1.9 ± 0.06 | 2.4 |
a
Three days postinfection, cells were pulsed with [8-3H]ACV (200 μM) or [8-3H]GCV (20 μM) for 18 h. Intracellular anabolites were measured by anion-exchange HPLC.
b
Values are the means ± standard deviations for three samples.