Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 24;42(4):428–443. doi: 10.1161/ATVBAHA.121.317365

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

PMC Copyright notice

Figure 5. — Upregulation of transcription factors that are involved in adipogenic differentiation: PPARγ (peroxisome proliferator activated receptor gamma), C/EBPα (CCAAT enhancer binding protein alpha), and C/EBPβ (CCAAT enhancer binding protein beta). A, Network of the predicted master regulators that are involved in transcription regulation. The interactions between the nodes are based on Qiagen ingenuity knowledge base, where the solid and dashed lines represent direct and indirect interaction, respectively. The intensity of coloring is based on the magnitude of Z score values ranging from strong inhibition (dark green) to strong activation (dark red). B, The top 13 master regulators and their fold change and Z score, which reflects their activation status. C, Quantification of Western blot analysis of PPARγ, C/EPBα, and C/EPBβ and representative blots to the right (Ctrl [control], n=5–6; Y/T KO [YAP/TAZ (yes-associated protein 1/WW domain containing transcription regulator 1) knockout], n=5–6). D, Quantification and representative blot showing PPARγ in the nuclear fraction and histone H3 as nuclear marker (Ctrl, n=6; Y/T KO, n=6). E, Alizarin Red staining for calcium deposits detection in aortae of Ctrl and Y/T KO mice. Inset represents positive Ctrl (bone). F, Assessment of lipid accumulation in the aorta by Oil Red O staining, positive staining in perivascular fat (arrow). Detailed legend description for IPA network is available online: https://qiagen.secure.force.com/KnowledgeBase/articles/Knowledge/Legend. All data are presented as mean±SEM. Ctrl indicates control; DAPI, 4′,6-diamidino-2-phenylindole; FC, fold change; and KO, knockout.