Fig 7. Carfilzomib Prevents GBS Brain Entry in vivo.

(A) Schematic generated using BioRender displaying the experimental design and injection/infection timepoints for the comparison of Carfilzomib or vehicle (10% DMSO in sesame oil) treatments. Mice were infected with ~8.5x10⁸ CFU of either WT or ΔbspC GBS. Four WT GBS infected vehicle treated mice and three WT infected carfilzomib treated mice were euthanized early based disease severity. All remaining mice were euthanized at 72 hours post infection, at which time the brain tissues (B) and blood (C) were harvested for enumeration of bacterial CFU. Pooled data from two independent experiments is shown. Error bars indicate standard error of the mean. Statistical analysis: (A) Two-way ANOVA with Tukey’s multiple comparisons. *, P < 0.05.