TABLE 2.
Kis of N-3-substituted TMAUs and EMAUs against the pol IIIs of B. subtilis, S. aureus, and E. faecalis
| AU | R group substituted at N-3 |
Ki (μM)a
|
||
|---|---|---|---|---|
| B. subtilis | S. aureus | E. faecalis | ||
| TMAU | -H | 1.20 | 1.59 | 1.22 |
| -CH3 (methyl) | 1.34 | 1.22 | 0.84 | |
| -CH2CH3 (ethyl) | 0.31 | 0.38 | 0.31 | |
| -CH2CH⩵CH2 (allyl) | 0.38 | 0.54 | 0.24 | |
| -(CH2)2CH3 (propyl) | 0.31 | 0.20 | 0.22 | |
| -(CH2)3CH3 (butyl) | 0.09 | 0.10 | 0.09 | |
| -(CH2)5CH3 (hexyl) | 0.24 | 0.42 | 0.14 | |
| -CH2C6H5 (benzyl) | 21.4 | 36.1 | 17.6 | |
| EMAU | -H | 1.00 | 1.16 | 0.61 |
| -CH2CH3 (ethyl) | 0.12 | 0.22 | 0.07 | |
| -CH2CH⩵CH2 (allyl) | 0.21 | 0.32 | 0.19 | |
a
All Kis are the averages of three independent experiments performed as described in the Materials and Methods section.