TABLE 4.
Inhibitor constants (Kis) of N-3 hydroxyalkyl and methoxyalkyl EMAU derivatives against relevant DNA polymerases
| R group |
Ki (μM)
|
||||
|---|---|---|---|---|---|
| B. subtilisb | S. aureusb | E. faecalisb | E. colib | pol alphac | |
| -(CH2)2OH (HE) | 0.59 | 2.48 | 0.48 | >160 | >160 |
| -(CH2)2OCH3 (ME) | 0.58 | 2.82 | 0.46 | >160 | >160 |
| -(CH2)3OH (HP) | 0.73 | 2.10 | 0.61 | >160 | >160 |
| -(CH2)3OCH3 (MP) | 1.00 | 1.27 | 0.42 | >160 | >160 |
a
All Kis are the averages of three independent experiments performed as described in the Materials and Methods section.
b
Denotes the DNA pol III of the indicated organism; see Materials and Methods section for source.
c
Derived from calf thymus; see Materials and Methods section for source.