Table 1. Summary of candidate and unbiased approaches to reveal Hoxc8 target genes in mouse brachial MNs.
| Gene name | Expression in WT brachial MNs | Hoxc8 dependency | |||||
|---|---|---|---|---|---|---|---|
| e12 | p8 | p56 Allen Brain ISH | p60snRNA-Seq dataset | Hoxc8 MNΔ early mice | Hoxc8 MNΔ late mice | ||
| Candidate approach | Slc10a4 | + | + | + | + | No | N.D |
| Nrg1 | + | + | + | + | Yes | Yes | |
| Nyap2 | + | + | N.D | + | No | N.D | |
| Sncg | + | + | + | + | No | N.D | |
| Ngfr | + | + | + | – | No | N.D | |
| Glra2 | + | + | + | + | No | Yes | |
| Cldn1 | N.D | – | N.D | – | N.D | N.D | |
| Cacna1g | N.D | – | + | + | N.D | N.D | |
| RNA-Seq approach | Slc44a5 | + | + | + | + | No | N.D |
| Mcam | + | + | + | + | Yes | Yes | |
| Pappa | + | + | + | + | Yes | Yes | |
| Sema5a | + | + | N.D | + | Yes | N.D | |
| Pex14 | + | + | N.D | + | No | N.D | |
| Tagln2 | + | + | + | – | No | N.D | |
| Cldn19 | N.D | – | – | – | N.D | N.D | |
| Wwc2 | N.D | – | + | + | N.D | N.D | |
| Septin1 | N.D | – | N.D | N.D | N.D | N.D | |
| Irx2 | + | + | + | – | N.D | N.D | |
| Irx5 | + | + | + | – | N.D | N.D | |
| Irx6 | + | + | + | – | N.D | N.D | |
| Known Hoxc8 targets | Ret | + | + | N.D | + | Yes | No |
| Gfra3 | + | – | – | – | Yes | N.D | |
Expression in p56 brachial MNs was determined using the Allen Brain Map (http://portal.brain-map.org). We also interrogated the single nucleus (sn) RNA-seq datasets of p60 spinal MNs from http://spinalcordatlas.org/.
N.D: Not determined; + denotes expression; – denotes no expression.
RNA-Seq: RNA-sequencing.